w v acrylamide linear gradient gel slabs Search Results


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Image Search Results


Journal: iScience

Article Title: Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology

doi: 10.1016/j.isci.2023.106657

Figure Lengend Snippet:

Article Snippet: BP-PA hydrogel precursor solution contained 3–7.5% acrylamide (40% w/v Am stock, 1610142, BioRad), 0.035–0.25% N,N-methylenebisacrylamide crosslinker (2% w/v Bis stock, 1610140, BioRad), 0.06% SDS (5% w/v stock in DI water; 161–0301, Bio-Rad), 0.06% Triton X-100 (5% w/v stock in DI water; T9284, Sigma), and 3 mM N-[3-[(4-benzoylphenyl) formamido]propyl] methacrylamide (BPMAC, custom synthesis, PharmAgra) and 10x Dulbecco’s phosphate buffered saline (DPBS; 14200075, ThermoFisher), diluted to a 1x final DPBS concentration in DI water.

Techniques: Recombinant, Staining, Plasmid Preparation, Software, Cell Culture